Rapid fluorescent-antibody staining technique.

Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining

In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water samples collected from 30 bathing facilities, with similar sensitivity of a conventional plate-count...

Specific staining of various bacteria with a single fluorescent antiglobulin.

Coons and Kaplan (1950) developed a rapid, sensitive, and specific fluorescent antibody technique for staining viruses in tissue sections. They used a direct staining procedure in which a specific fluorescent antibody preparation is applied directly to the cell being studied. In the indirect procedure (Weller and Coons, 1954), the virus is first reacted with a specific nonfluorescent antiserum ...

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Rapid fluorescent-antibody conjugation procedure.

This note describes a technique developed for rapid preparation of specific and brilliant conjugates for direct staining of rickettsial agents in yolk-sac, tick, and vertebrate tissues.

Agar block technique for identification of mycoplasmas by use of fluorescent antibody.

A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of myc...